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Study participant in front of a Tobii Pro Spectrum eye tracker, four visual stimuli on display; caption: Before you hit record
⚡ Interlab Methodological Guide · Research practice

Eye-tracking data quality — how to prepare a study

Six steps to control calibration, accuracy, precision, and session workflow before an issue affects the entire dataset.

A study where calibration drift is discovered only after recruitment ends does not simply yield “slightly worse” results. Part of the recording may be unassignable to areas of interest, requiring sessions to be repeated. The cost of such an error includes participant time, team labor, and re-running the protocol.

This can be prevented if data quality is treated not as a feature of the purchased device, but as the outcome of a reproducible procedure. Below, we distinguish between two frequently confused concepts, then walk through the environment, equipment, participant, calibration, piloting, and post-session control. These decisions determine whether a recording will be reliable and suitable for analysis.

1. Two concepts that must be kept separate

Accuracy according to the Tobii glossary, is the average angular distance between the true gaze point and the point measured by the eye tracker. It primarily describes systematic error: the measurement may be stable, but consistently offset from the target location. Precision describes the spatial angular variability between individual consecutive samples, calculated on raw data using the RMS method. It is a measure of spread, or measurement noise.

🎯 Interactive Accuracy and Precision Simulator (Target Simulator)

Test live how changing offset and noise affects sample distribution around the target:

Accuracy Error (Systematic offset):0.30°

Shifts the centroid of the sample cluster away from the target center.
Precision Noise (RMS sample spread):0.01°

Scatters individual samples around the centroid (measurement noise).
Laboratory takeaway: Samples are tightly clustered (high precision) and lie very close to the target (high accuracy).

Datasheet specifications describe capabilities achieved under specific testing protocols. Values “under optimal conditions” represent a ceiling, not a guarantee of performance in a given laboratory setting. Therefore, they must be interpreted alongside head positioning, filtering methods, and precision calculation techniques.

Device Accuracy Precision
Tobii Pro Spectrum median 0.30° under optimal conditions, with head support 0.01° RMS under optimal conditions, S-G filter
Tobii Pro Fusion 0.3° under optimal conditions 0.04° RMS under optimal conditions, S-G filter
Tobii Pro Spark 0.45° under optimal conditions 0.26° RMS under optimal conditions
Tobii Pro Glasses 3 0.6° under optimal conditions not specified by the manufacturer
Tobii Glasses X 1.5°–2.5° under optimal conditions not specified by the manufacturer

2. Step one: define the study environment

Preparation begins before unwrapping cables. In screen-based studies, stable ambient light must be ensured without harsh reflections or shifting patches of direct sunlight. Conditions must remain comparable across participants, as changes in reflection geometry and pupil size can affect gaze mapping.

An easily overlooked detail: remove fingerprints and smudges from the display. They reflect infrared light and can degrade tracking conditions, even if they appear harmless to the operator.

Tobii Pro Spark mounted below the screen

Tobii Pro Spark mounted centered below the monitor. (Click image to enlarge)
Tobii Pro Spectrum controlled workstation

Controlled workstation with chin rest and constant illumination. (Click image to enlarge)
Calibrating Tobii Pro Glasses 3 wearable glasses with a card

Field study with Tobii Pro Glasses 3: lighting survey of the route prior to the session. (Click image to enlarge)
Screen-based eye-tracking workstation control view — geometry schematic, trackbox, and lighting
Screen-based workstation control view: Document the participant and tracker geometry and check the infrared light path up to the display reflection point. (Click image to enlarge)

1 Desk & Monitor

Centered eye tracker position below the bottom bezel. Screen tilt angle compliant with device specifications.

2 Light Direction & Reflections

Ensure constant illumination without moving sunlight patches. Verify the absence of infrared reflections on the display surface.

3 Head-movement Box Boundary

Trapezoidal tracking volume keeping the participant’s head inside the usable operating region (55–75 cm for Spectrum).

4 Participant Position & Chair

Stable posture, comfortable chair in a reproducible position without accidental movement after calibration.

3. Steps two and three: prepare the equipment and participant

For screen-based systems, the setup workflow is straightforward: unpack, connect, align, and power on. The tracker must be mounted according to model guidelines and centered relative to the participant’s eyes. Then, in Tobii Pro Lab, verify device recognition and inspect the live eye view.

📏 Interactive Distance and Trackbox Tolerance Calculator

Adjust the slider for the participant’s distance from the display to check eye-tracking tolerance:

Set participant distance from sensors:60 cm

✅ Optimal distance! Fits Tobii Spectrum (55–75 cm), Fusion (50–80 cm), and Spark (45–95 cm).
Researcher positioning a participant in front of a screen-based eye tracker

Participant posture dictates data quality more than hardware specs. · Context: screen-based eye-tracking session. Source: Tobii. (Click image to enlarge)

4. Step four: calibration and validation

Calibration fits an eye model to an individual participant, but merely completing the procedure is insufficient. In Tobii Pro Lab, the workflow includes point collection, optimization, and separate validation.

🧪 Validation Decision Simulator in Tobii Pro Lab

Test two validation report scenarios before starting formal recording:

Accuracy (Offset):
0.23°
Precision (RMS):
0.28°
Gaze Samples:
94%
✅ OPERATOR DECISION: Project thresholds met. Proceed to start the main session!
Calibration data window in Tobii Pro Lab

Original Calibration Data window in Tobii Pro Lab: an X marks four validation points located at different coordinates than calibration points. (Click image to enlarge)

5. Step five: run a complete pilot session

Rule number one

“Never run your first session with a participant you’re paying for or scheduling formally.”

The first session is a pilot with a team member, not a formal participant recording. — Tobii, Eye tracking setup: A step-by-step guide for reliable results.

The pilot run should reproduce the entire study workflow, not merely confirm that the device saves a file. Walk through instructions, calibration, stimulus presentation, tasks, event triggers, and session termination.

6. Step six: run, check, repeat

A successful pilot does not eliminate the need to review actual sessions. Inspect a brief segment of data in Tobii Pro Lab immediately after completion.

📊 Gaze Samples Health Indicator Simulator

Adjust the gaze samples slider from the recording list to view moderator recommendations:

Gaze Samples column value in Tobii Pro Lab:89%

🟢 HIGH PROPORTION OF VALID SAMPLES (89%): An indicative result. Readiness for AOI analysis also depends on the protocol, population, task, and distribution of missing data.
Recording list in Tobii Pro Lab with Gaze samples column

Recording list in Tobii Pro Lab with Gaze samples column (78%, 77%, 89%, 90%). (Click image to enlarge)

7. What calibration cannot fix

AOI boundaries and a margin derived from system accuracy. With adjacent areas of interest and accuracy of 0.2–0.5° in screen-based systems or from 0.6° in wearable setups, assigning a fixation lying on an edge can be ambiguous.

🔍 Margin of Error Simulator for Adjacent AOIs

No margin (0.0°) — Error Risk!

See how accuracy error (0.4°) causes fixations to spill into adjacent buttons without a safety margin (~0.5°):

BUY NOW (AOI 1)
DETAILS (AOI 2)
AOI editor in Tobii Pro Lab

AOI editor in Tobii Pro Lab: regions abut each other without a safety margin. (Click image to enlarge)

8. Summary: operator procedure

Pre-session operator checklist

0 / 5 completed

Interactive checklist for lab station protocol:

1. EnvironmentStable lighting, clean screen, no glare or line-of-sight obstructions.

2. EquipmentTracker detected, valid dual-eye tracking preview in Tobii Pro Lab, clean lenses.

3. ParticipantNatural posture, correct distance (55–65 cm), fitted nose pads, and fixed chair position.

4. ValidationAccuracy, precision, and valid sample percentage meet protocol thresholds.

5. Recording CheckShort data segment reviewed post-session; adjustments logged before the next participant.

Data Quality Glossary

Trackbox

Head movement volume in which at least one eye remains within the eye tracker’s field of view.

Operating distance

Minimum and maximum distance between eyes and sensors maintaining stable tracking.

Blink recovery time

Tracking recovery duration following brief pupil occlusion when head position remains unchanged.

Gaze recovery time

Gaze re-acquisition latency following tracking loss; under 150 ms for Pro Spectrum.

Eye openness

Diameter of the largest sphere inscribed between eyelids, reported in millimeters per eye.

Binocular and Monocular Calibration

Binocular mode processes data from both eyes independently; monocular optimizes the eye model specifically for a single eye.

Read further

Author and technical review
Head of the Physiology Division at INTERLAB, certified Tobii trainer. Delivers eye-tracking technology training and implements behavioural research laboratories.
Last technical review: 3 August 2026.

Sources

  1. Tobii — Eye tracking setup: A step-by-step guide for reliable results — six steps for session preparation and control.
  2. Tobii Connect — Eye tracker calibration and validation — calibration phases, validation, and quality report.
  3. Tobii Connect — Tobii eye tracker glossary — definitions of accuracy, precision, and tracking parameters.